Fibrin has been used extensively in cell encapsulation because it has important biological properties. Keratinocyte encapsulation in fibrin is a widely used technique in skin tissue engineering. The production of growth factors (EGF, TGF-β1 and PDGF-BB) was evaluated when keratinocytes are encapsulated in fibrin. Secretions of TGF-β1 and PDGF-BB increased more than five times compared to monolayer cultures. Encapsulated cells secreted about 80% active form of TGF-β1 (monolayer cells only secreted inactive form). An enhanced secretion of TGF-β1 and PDGF-BB was found in encapsulated cells, showing that fibrin capsules are favourable for the production of these growth factors. © 2010 Springer Science+Business Media B.V.
Acevedo, C. A., Somoza, R. A., Weinstein-Oppenheimer, C., Brown, D. I., & Young, M. E. (2010). Growth factor production from fibrin-encapsulated human keratinocytes. Biotechnology Letters, 1011-1017. https://doi.org/10.1007/s10529-010-0245-5