Growth factor production from fibrin-encapsulated human keratinocytes

Cristian A. Acevedo, Rodrigo A. Somoza, Caroline Weinstein-Oppenheimer, Donald I. Brown, Manuel E. Young

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Fibrin has been used extensively in cell encapsulation because it has important biological properties. Keratinocyte encapsulation in fibrin is a widely used technique in skin tissue engineering. The production of growth factors (EGF, TGF-β1 and PDGF-BB) was evaluated when keratinocytes are encapsulated in fibrin. Secretions of TGF-β1 and PDGF-BB increased more than five times compared to monolayer cultures. Encapsulated cells secreted about 80% active form of TGF-β1 (monolayer cells only secreted inactive form). An enhanced secretion of TGF-β1 and PDGF-BB was found in encapsulated cells, showing that fibrin capsules are favourable for the production of these growth factors. © 2010 Springer Science+Business Media B.V.
Original languageEnglish
Pages (from-to)1011-1017
Number of pages7
JournalBiotechnology Letters
DOIs
Publication statusPublished - 1 Jul 2010

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